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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
29/09/2014 |
Actualizado : |
24/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
ISSN : |
0717-3458 |
DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
Thesagro : |
CITRUS; CYDIA. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02405naa a2200241 a 4500 001 1050705 005 2021-06-24 008 2012 bl uuuu u00u1 u #d 022 $a0717-3458 024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI 100 1 $aFEDERICI, M. 245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource] 260 $c2012 520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. 650 $aCITRUS 650 $aCYDIA 700 1 $aMARCONDES, J.A. 700 1 $aPICCHI, S.C. 700 1 $aSTUCHI, E.S. 700 1 $aFADEL, A.L. 700 1 $aLAIA, M.L. 700 1 $aLEMOS, M.V.F. 773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
06/11/2014 |
Actualizado : |
08/03/2021 |
Tipo de producción científica : |
Poster |
Autor : |
FÉLIX, A.; REPETTO, J.L.; HERNÁNDEZ, N.; PERDOMO, I.; SILVERA, R.; PÉREZ, A.; AGUERRE, M.; MENDOZA, A.; CAJARVILLE, C. |
Afiliación : |
JOSE LUIS REPETTO CAPELLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Efecto de restringir el tiempo de acceso al forraje fresco sobre el perfil de hormonas y metabolitos sanguíneos en terneras. |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
In: JORNADAS URUGUAYAS DE BUIATRÍA, 41, 2013, Paysandú, Uruguay. Posters. Paysandú, UY: Centro Médico Veterinario de Paysandú, SUB, SMVU. 2013. |
Páginas : |
P. 186-187. |
Idioma : |
Español |
Palabras claves : |
GLUCAGÓN; GLUCOSA; INSULINA; UREA. |
Thesagro : |
NUTRICIÓN ANIMAL. |
Asunto categoría : |
L51 Fisiología Animal - Nutrición |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15301/1/JB2013-186-187.pdf
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Marc : |
LEADER 00851nam a2200265 a 4500 001 1051542 005 2021-03-08 008 2013 bl uuuu u00u1 u #d 100 1 $aFÉLIX, A. 245 $aEfecto de restringir el tiempo de acceso al forraje fresco sobre el perfil de hormonas y metabolitos sanguíneos en terneras. 260 $aIn: JORNADAS URUGUAYAS DE BUIATRÍA, 41, 2013, Paysandú, Uruguay. Posters. Paysandú, UY: Centro Médico Veterinario de Paysandú, SUB, SMVU. 2013.$c2013 300 $aP. 186-187. 650 $aNUTRICIÓN ANIMAL 653 $aGLUCAGÓN 653 $aGLUCOSA 653 $aINSULINA 653 $aUREA 700 1 $aREPETTO, J.L. 700 1 $aHERNÁNDEZ, N. 700 1 $aPERDOMO, I. 700 1 $aSILVERA, R. 700 1 $aPÉREZ, A. 700 1 $aAGUERRE, M. 700 1 $aMENDOZA, A. 700 1 $aCAJARVILLE, C.
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